TU Wien:Grundlagen der Zellkultur VO (Sevcsik)

Daten

Vortragende	Marica Markovic• Mina Petrovic• Eva Sevcsik
ECTS	1,5
Alias	Mammalian Cell Culture (en)
Letzte Abhaltung	2024S
Sprache	English
Mattermost	grundlagen-der-zellkultur • Register • Mattermost-Infos
Links	tiss:134231

Zuordnungen

Masterstudium Biomedical Engineering
Katalog Freie Wahlfächer

Inhalt

noch offen, bitte nicht von tiss oder Homepage kopieren, sondern aus Studierendensicht beschreiben.

Die Lehrveranstaltung - respektive die Folien - geht/gehen in verschiedene Grundlagen der Zellkultur ein: steriles Arbeiten, Faktoren die für die Zellkulturen wichtig sind, Kontaminationen und wie man diese vermeidet und ähnliche Themen. Sie ist für die assoziierte praktische Übung konzipiert; beide sind jedoch von der ECTS Zahl sehr eingeschränkt und eher eine Art "crash course" (da relativ wenig Zeit verfügbar ist; die LU ist innerhalb von 2 Tagen erledigt).

At the begging of each lecture the professors quickly summarizied the last lecture by some questions:

The fourth and sixth lecture were each the last lecture of the professors, which is why there are no summary questions from them, so here I wrote what I think is important to know.

What should you know?

Part 1:

• What is cell culture and why do we do it?
• What do cells need to grow ex vivo?
• What equipment do you need for cell culture?
• What is aseptic technique?

Part 2:

• What are biosafety levels and what are they good for?
• Biosafety level 2: What should a lab have to run?
• What is cell proliferation? Do cells proliferate indefinitely ? (Why not?)
• Advantages and disadvantages of primary cells and cell line
• Categories of cultured cells

Part 3:

• How to maintain cultured cells?
• Steps involved in passaging adherent/suspension cells
• What to take care of when freezing/thawing cells?

Part 4:

• Transfection
  • Stable Transfection
  • Transient Transfection
• Transfection Method 1: Chemical transfection (transient)
• Transfection Method 2: Physical transfection (transient)
• Transduction Method 1: Retro/lentiviral (RNA virus)
• Transduction Method 2: Adenoviral (DNA virus)
• Cell differentiation
• Cell dedifferentiation

Part 5:

• How can I quantify the number of cells in one sample?
• What is surrounding the cells?
• What is tripo assay?
• At which level can I study the behavior of cells?

Part 6:

• not sure, but maybe like the main assays

Ablauf

noch offen

Benötigte/Empfehlenswerte Vorkenntnisse

None, because it is about basics of cell culture.

Vortrag

noch offen

Übungen

Für die assoziierte Übung ist die VO Voraussetzung.

Prüfung, Benotung

22.05.2024 exam

1. What is the difference between adhesion and suspension cells? Give one example for each? When do you make subcultivation.
2. What is cell differentation? Can it be reversed? If yes, how? If no, why?
3. What is the principle of Live/Dead assay?
4. What are advantages of 3D cell culture?
5. What equipment have you for Biosafety 2?
6. What are immortalized cells? Give example and how can you obtain them?
7. True/False as on exams before:
   1. Adenoviral transduction is not integrating in genome - sth like this.

Dauer der Zeugnisausstellung

Less than 1 week.

Zeitaufwand

noch offen

Unterlagen

• Materialien

Tipps

Learn more than just the content of "What should you know?", as you can see under "Materialien", some exam questions are not listend there.

Highlights / Lob

noch offen

Verbesserungsvorschläge / Kritik

noch offen

Materialien

Neues Material hinzufügen

1

• 134.231 Grundlagen der Zellkultur Exam Questions on the 13.12.2022.pdf (details)
• 134.231 Grundlagen der Zellkultur.apkg (details)

E

• Exam 05.05.23.pdf (details)
• Exam 06.05.2024
• Exam 15.05.23.pdf (details)
• Exam 2024-05-22

M

• Mammalian Cell Culture Exam 20231205.pdf (details)