TU Wien:Grundlagen der Zellkultur VO (Sevcsik)

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Vortragende Marica Markovic• Mina Petrovic• Eva Sevcsik
ECTS 1,5
Alias Mammalian Cell Culture (en)
Letzte Abhaltung 2025S
Sprache English
Mattermost grundlagen-der-zellkultur • Register • Mattermost-Infos
Links tiss:134231
Zuordnungen
Masterstudium Biomedical Engineering Modul Modul: Advances in Medical Physics & Imaging
Katalog Freie Wahlfächer


Inhalt[Bearbeiten | Quelltext bearbeiten]

noch offen, bitte nicht von tiss oder Homepage kopieren, sondern aus Studierendensicht beschreiben.

Die Lehrveranstaltung - respektive die Folien - geht/gehen in verschiedene Grundlagen der Zellkultur ein: steriles Arbeiten, Faktoren die für die Zellkulturen wichtig sind, Kontaminationen und wie man diese vermeidet und ähnliche Themen. Sie ist für die assoziierte praktische Übung konzipiert; beide sind jedoch von der ECTS Zahl sehr eingeschränkt und eher eine Art "crash course" (da relativ wenig Zeit verfügbar ist; die LU ist innerhalb von 2 Tagen erledigt).

At the begging of each lecture the professors quickly summarizied the last lecture by some questions:

The fourth and sixth lecture were each the last lecture of the professors, which is why there are no summary questions from them, so here I wrote what I think is important to know.

What should you know?

Part 1:

  • What is cell culture and why do we do it?
  • What do cells need to grow ex vivo?
  • What equipment do you need for cell culture?
  • What is aseptic technique?

Part 2:

  • What are biosafety levels and what are they good for?
  • Biosafety level 2: What should a lab have to run?
  • What is cell proliferation? Do cells proliferate indefinitely ? (Why not?)
  • Advantages and disadvantages of primary cells and cell line
  • Categories of cultured cells

Part 3:

  • How to maintain cultured cells?
  • Steps involved in passaging adherent/suspension cells
  • What to take care of when freezing/thawing cells?

Part 4:

  • Transfection
    • Stable Transfection
    • Transient Transfection
  • Transfection Method 1: Chemical transfection (transient)
  • Transfection Method 2: Physical transfection (transient)
  • Transduction Method 1: Retro/lentiviral (RNA virus)
  • Transduction Method 2: Adenoviral (DNA virus)
  • Cell differentiation
  • Cell dedifferentiation

Part 5:

  • How can I quantify the number of cells in one sample?
  • What is surrounding the cells?
  • What is tripo assay?
  • At which level can I study the behavior of cells?

Part 6:

  • 3D Cell Cultures

Ablauf[Bearbeiten | Quelltext bearbeiten]

Six lectures, approximately 45 to 60 minutes per session. The first possibility to take the final exam is one week after the last lecture.

Benötigte/Empfehlenswerte Vorkenntnisse[Bearbeiten | Quelltext bearbeiten]

None, because it is about basics of cell culture.

Vortrag[Bearbeiten | Quelltext bearbeiten]

I liked it. It was easy to follow and required no previous knowledge. It helps if you've heard about the basics of cells and just some biology in general before attending the lectures, but it's not necessary for attending the course.

Übungen[Bearbeiten | Quelltext bearbeiten]

Für die assoziierte Übung ist die VO Voraussetzung.

Prüfung, Benotung[Bearbeiten | Quelltext bearbeiten]

09.05.2025 exam

Overall similar to the previous exams; Only 1-2 questions were different

  1. What is the difference between adhesion and suspension cells? Give one example for each? When do you make subcultivation.
  2. What is cell differentation? Can it be reversed? If yes, how? If no, why?
  3. What are immortalized cells? Give example and how can you obtain them?
  4. Explain some methods of performing transfection. Give examples
  5. What type of flask would you use for cultivating HeLa cells
  6. What assays are available for testing the cytocompatibility of biomaterials
  7. True/False Questions

22.05.2024 exam

  1. What is the difference between adhesion and suspension cells? Give one example for each? When do you make subcultivation.
  2. What is cell differentation? Can it be reversed? If yes, how? If no, why?
  3. What is the principle of Live/Dead assay?
  4. What are advantages of 3D cell culture?
  5. What equipment have you for Biosafety 2?
  6. What are immortalized cells? Give example and how can you obtain them?
  7. True/False as on exams before:
    1. Adenoviral transduction is not integrating in genome - sth like this.

Dauer der Zeugnisausstellung[Bearbeiten | Quelltext bearbeiten]

Semester Letzte Leistung Zeugnis
SS25 07.05.2025 09.05.2025 2 days

Zeitaufwand[Bearbeiten | Quelltext bearbeiten]

About 10h for visiting the lectures; About 8-12h preparation for the exam

Unterlagen[Bearbeiten | Quelltext bearbeiten]

Tipps[Bearbeiten | Quelltext bearbeiten]

Learn more than just the content of "What should you know?", as you can see under "Materialien", some exam questions are not listend there.

Highlights / Lob[Bearbeiten | Quelltext bearbeiten]

noch offen

Verbesserungsvorschläge / Kritik[Bearbeiten | Quelltext bearbeiten]

noch offen

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